Understanding Glyph Separation:
When calculating planet positions, it is possible that any number of planets will fall closely together in a small span of the zodiac. If glyphs were nothing more than dots on a screen, plotting them without obscuring some of the glyphs would rarely be a problem. However because a glyphs font size (angular width from the left to the right) is often 3 or more degrees, when 2 planets are closer together than this, one of the glyphs will overwrite the other and make it less visible or legible.
The glyph separation value represents the minimum number of degrees in zodiacal longitude that must separate one glyph from the next.
On the uni-wheel tab, there is a glyph separation edit field where you can enter an angular value between 0 and 6 degrees. Any decimal (real number) value is allowed within this range. Values above 6 would rarely be needed (and would not look very good) - and only if the user is running an extremely small monitor resolution (say 640 x 480).
When setting the glyph separation value, you should consider your screen size and other considerations to get the best balance between accuracy and readability.
It is important to realize that the glyph separation values in the chart designer and in the Html Report Options are independent from eachother. They represent the same thing, but you will not necessarily have the same screen setup for your gif files as you will for your main display which will usually be larger.